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mcdb 131 medium  (Thermo Fisher)


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    Structured Review

    Thermo Fisher mcdb 131 medium
    Mcdb 131 Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mcdb+131+medium/D(%2B)-Glucose/bio_rxiv__2025__09__26__677825-146-9-12
    Average 99 stars, based on 1 article reviews
    mcdb 131 medium - by Bioz Stars, 2026-09
    99/100 stars

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    Cell Culture:

    Article Title: The role of IGF-2 and its variants in enhancing endothelial migration and angiogenesis
    Article Snippet: The human microvascular endothelial cell-1 (HMEC-1) line was procured from the Centers for Disease Control and Prevention [CLS Cat# 304064, RRID:CVCL_0307 , Atlanta, GA, United States, ( )]. .. Cells were cultured in standard HMEC-1 growth medium comprising MCDB 131 medium (10372-019, Gibco, Paisley, United Kingdom) supplemented with 10% heat-inactivated fetal bovine serum (FBS, s181B-500, Biowest, Nuaillé, France), 100 U/ml Penicillin and 100 μg/ml Streptomycin (1% P/S, P4333, Sigma-Aldrich, Saint Louis, MO, United States), 10 mM L-glutamine (G7513, Sigma-Aldrich, Saint Louis, MO, United States), 10 ng/mL human epidermal growth factor (EGF, PGH0311L, Gibco, Paisley, United Kingdom) and 1 μg/ml hydrocortisone (A16292.03, Alfa Aesar, Thermo Fisher Scientific, Haverhill, MA, United States). ..

    Article Title: Caspase-Driven Microvascular Inflammation and Hypoperfusion in Intravascular Hemolysis: Roles of Leukocyte and Endothelial Activation
    Article Snippet: HUVECs were cultured in modified Ham’s F-12 Kaighn’s medium (F12K, Gibco) with 10% fetal bovine serum (FBS, Invitrogen, MA, USA), endothelial growth factor (30 ng/mL, Calbiochem, CA, USA), sodium heparin (10 U/mL, Cristália, São Paulo, Brazil), and antibiotics (streptomycin and penicillin). .. HMEC-1 were cultured in MCDB 131 medium (Gibco) with 10% FBS, 10 ng/mL Epidermal Growth Factor (EGF, Thermofisher, MA, USA), 1 μg/mL hydrocortisone (Sigma-Aldrich), 10 mM L-Glutamine (Gibco), and antibiotics. ..

    Article Title: Subcutaneous liposomal delivery improves monoclonal antibody pharmacokinetics in vivo.
    Article Snippet: .. HMEC-1 and HMEC-1 FcRn were cultured in MCDB 131 medium (Thermo Fisher) supplemented with 10 % fetal bovine serum, 100 U/ml penicillin, 100 μg/ml streptomycin, 1 μg/ml hydrocortisone (Sigma-Aldrich, St. Louis, MO), 10 ng/ml Epidermal Growth factor (EGF; Thermo Fisher) and 1 mg/ml G418. .. Antibiotic-resistant cells were further screened for transgene expression using fluorescence-activated cell sorting using a BD FacsAriaII cell sorter (BD Biosciences, San Diego, CA) and maintained in selective media for subsequent experiments.

    other:

    Article Title: The FKBPL-based therapeutic peptide, AD-01, protects the endothelium from hypoxia-induced damage by stabilising hypoxia inducible factor-α and inflammation.
    Article Snippet: Human microvascular endothelial cells (HMEC-1) obtained from the Centre for Disease Control and Prevention (MTA M1224I) were maintained in MCDB 131 medium (Gibco, USA) supplemented with 10% foetal bovine serum (FBS, Bovogen biologicals, SFBS-AU), epidermal growth factor (0.01 μg/mL, Life technologies, cat. #PHG6045), penicillin/streptomycin (5 U/mL; Sigma Aldrich, P433), L-glutamine (2 mmol/L; Thermo Fisher, cat. #325030081) and hydrocortisone (500 μg/mL; Sigma Aldrich, cat. #H0888).

    Article Title: The IsletTester Mouse: An Immunodeficient Model With Stable Hyperglycemia for the Study of Human Islets.
    Article Snippet: All stage-specific base media were comprised of MCDB 131 medium (Thermo Fisher Scientific, #10372-019) supplemented with NaHCO3 (Sigma-Aldrich, #S6297), For Peer Review Only Diabetes D ow nloaded from http://diabetesjournals.org/diabetes/article-pdf/doi/10.2337/db23-0887/789900/db230887.pdf by Viet N am Institution user on 03 D ecem ber 2024



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